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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Paederoside Promotes Longevity and Fitness in C. elegans Through Ubiquitination and Degradation of DAF-2/IGF1R, Activating DAF-16/FOXO and SKN-1/NRF2 Transcription Factors
doi: 10.3390/ijms27052248
Figure Lengend Snippet: ( a ) Molecular docking outcomes. Western blot analysis of ( b ) ubiquitination, ( c ) IGF1R, ( d ) CoIP and ( f ) key proteins of the IIS pathway. ( e ) FOXO3 nuclear translocation assay. The levels of statistical significance were set at * p < 0.05, ** p < 0.01, and **** p < 0.0001, ns and nd indicates no statistical significance.
Article Snippet: The antibodies used in this study were as follows: β-actin (HUABIO, Hangzhou, China, 1:20,000, HA722023); β-Tubulin (HUABIO, Hangzhou, China, 1:20,000, EM0103); IGF1R (abcam, Cambridge, MA, USA, 1:1000, ab182408, d,f);
Techniques: Western Blot, Ubiquitin Proteomics, Nuclear Translocation Assay